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... Separation of a mixture of biomolecules by size exclusion chromatography Types of Column Chromatography Affinity Hydrophobic Interaction (HIC) Ion Exchange Anion Cation ...

... of Chromatography 1903 Tswett - plant pigments separated on chalk columns (radial chromatography) 1931 Lederer & Kuhn - LC of carotenoids 1938 TLC and ion exchange 1950 ...

Ion Exchange Chromatography 4. Gel Permeation Chromatography (exclusion chromatography) BASIC LIQUID CHROMATOGRAPHY LIQUID SOLID CHROMATOGRAPHY The separation ...

Dionex BioLC: General Positioning Separation of carbohydrates, amino acids, peptides, proteins, and nucleic acids by ion-exchange chromatography Importance of ...

Chromatographic Process Chromatographic Systems Chromatographic Techniques Chromatography – Separation Mechanism Adsorption Partition Ion - Exchange & Ion ...

... polystyrene support Mechanism RSO3-H+ + P RSO3-P+ + H+ Ion Exchange Gradients Mobile Phase A – H2O Mobile Phase B – 500 mM NaAc Ion chromatography ...

... wise home made work stations FPLC/HPLC HPLC = High Performance (Pressure) Liquid Chromatography FPLC = Fast Protein Liquid Chromatography Ion Exchange Chromatography (IEC ...

ION-EXCHANGE CHROMATOGRAPHY Separation in Ion-exchange Chromatography is based on the competition of different ionic compounds of the sample for the active sites on the ...

Ion Exchange Chromatography If the column is negatively charged charged i.e. carboxymethyl then…. Proteins with pIs l < the pH of the buffer will be negatively charged ...

Mechanism of Ion-Exchange Chromatography of Amino Acids SO 3 - SO 3 - Na + COO - H 3 N + Na + COOH H 3 N + pH2 pH4.5 Ion-exchange Resin Chromatography of Amino Acids Gel ...

Figure 3.7 DNA purification by ion-exchange chromatography. (a) Attachment of DNA to ion-exchange particles. (b) DNA is purified by column chromatography.

- Ion exchange resin Ion exchange - Microporous beads ... silica gel - water, wide range of solvents, limited pH Ion Exchange Chromatography ...

... performance (pressure flow) Thin layer (adsorption) Classification based on Attractive Forces Adsorption Ion Exchange Partition Size Exclusion Adsorption Chromatography ...

... Adsorption just on surface, partition into thin layer Not used as widely as partition used mainly in TLC & very small particles in LC Movie Ion Exchange Chromatography ...

Ion Exchange Laboratory Today’s Schedule Pre-lab discussion Ion Exchange and Spectrophotometer Ion exchange experiment Examples of Column Chromatography Ion ...

(Novagen) Ion Exchange Chromatography Globular Protein Deformation due to interaction with conventional ion exchanger Maintenance of conformation while interacting with ...

... isotachophoresis Capillary isoelectric focusing Capillary electrochromatography Micellar electrokinetic capillary chromatography Ion Exchange Chromatography ...

HPLC Analysis of Ionic Compounds Nicholas H. Snow Seton Hall University HPLC of Ionic Compounds Ion Suppression Ion Exchange Chromatography (IEX or IEC) Ion Pair ...

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