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Microbiology Practical I The following slides were contributed by your fellow BIO 251 students… Use side “ ” key to view slides… Lab 1: Eukaryotes and ...

MICROSCOPY AND STAINING. ... Simple Stain. Provide basic contrast for light compound microscope. ... Gram Stain. Crystal violet stain.

MICROSCOPY AND STAINING ... increases contrast Common stains Differential stains Gram Stain Gram Stain The single most important stain in microbiology.

Indirect staining with acidic dye (Negative staining) The negative stain technique does not stain the bacteria but stain the background. The bacteria will appear ...

Keep it zipped! Gram Stain of Urethral Discharge Urinanalysis Urine clear upon gross examination. Sample positive for leuckocytes esterase. Growth on SBA and EMB ...

The above is the bacteria used in the DNA extraction lab. Note its Spherical appearance and the color after gram staining. The gram stain specifically stains the cell ...

Gram Stain Procedure: The gram stain is the most commonly used differential stain for determining cell morphology. Differential stains allow for distinguishing ...

... and highly pleomorphic Don’t stain with a Gram’s stain Non-motile May possess a capsule Although some are free living, ...

Gram Staining. Gram + simple ... http://highered.mcgraw-hill.com/sites/007337525x/student_view0/exercise9/gram_stain.html. ... part of cell wall of gram – bacteria ...

... Watch Demo on how to make a smear for a indirect stain Examining Indirect Stains Example of a Negative Stain Gram Stain ASM MicrobeLibrary, ...

The Gram stain Thin smear/heat fix Gram stain: a. Flood slide with crystal violet and let stain for 1 minute. b. Drain off crystal violet and rinse off with distilled ...

... Cell Wall PowerPoint Presentation Gram-Negative Cell Wall PowerPoint Presentation Comparison of Gram-Positive and Gram-Negative The Gram Stain PowerPoint ...

Gram staining Types of staining Simple Bacteria stained to contrast environment Differential Bacteria differing chemically react differently to staining Gram stain ...

Steps of Gram Staining. Place a slide with a BACTERIAL smear . STAIN the slide with crystal violet for 1-2 min. Pour off the stain . Flood slide with Gram's IODINE ...

Developed by Christian Gram . He noticed that some bacteria retained dye while others washed out. Differential stain- shows differences among bacteria.

Gram Stain Procedure. Separates all bacteria into 2 groups: Gram Positive. Stain purple. Retain crystal violet. Gram Negative. stain pink or red. Retain safranin

... Periplasmic binding protein Gram stain Permeases Gram ... Cell Wall * GRAM POSITIVE GRAM NEGATIVE Cytoplasm Cytoplasm ...

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